Determination of median levels of the free β subunit of human chorionic gonadotropin in women from mainland China using a new time-resolved fluoroimmunoassay

Yong-Ping Tang, Ying-Song Wu, Ai-Hua Yin, Wei-Wen Xu, Peng-Hui Yuan & Ming Li
Background: The free β subunit of human chorionic gonadotropin (free β-hCG) is an important serum marker for biochemical screening. Its weekly median value varies with ethnicity. Most of the fluorometers for lanthanide chelates are designed for the detection of signals from europium (Eu3+) chelates only. Methods: We developed a two-site, one-step assay using two monoclonal antibodies (MAbs) against free β subunit and β subunit with Eu3+ chelates as labels. Using the present assay, we evaluated...
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