FLIM images in SPCImage Document format, originally analysed SPCImage from Becker & Hickl. Includes IRF. FLIM images with accompanying IRFs of: - HeLa cells stained with mitochondrial matrix targeting molecular rotor (control, nystatin and histamine conditions) - Extracted mitochondria stained with membrane targeting molecular rotor (high and low glucose growth conditions)Data supports Steinmark, E. 'Mitochondrial matrix viscosity and membrane fluidity are environmentally and physiologically responsive', PLOS One, Submitted.