An investigation into acid-base balance at the gills of rainbow trout (Oncorhynchus mykiss) using in situ hybridization and immunocytochemistry for proton-ATPase and band 3.

Gary V. Sullivan
This thesis examines changes that occur in the epithelial cells of rainbow trout (Oncorhynchus mykiss) gills with respect to the expression of Na$\sp+$ and Cl$\sp-$ ion-transporting proteins during periods of acid-base disturbance. In the first part of the study, the expression of the V-type proton ATP-ase (H$\sp+$-ATPase) was examined in the gill of trout to provide direct evidence for its existence, determine its cellular distribution, and assess its response to acid-base disturbance. Gill epithelial cells...
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