DNA dependent RNA polymerase activity of rat heart nuclei during experimentally induced hypertrophy.

Katalin. Varnai
Nuclei from groups of twelve pooled left ventricles of rats were isolated and DNA, RNA and protein contents of the nuclear suspension were determined. RNA polymerase activity was assayed in the presence of either Mg++ or Mn++ and (NH4)2SO4. Electronmicroscopic examination of normal nuclear preparations revealed undamaged nuclei with very little contamination. Cardiac enlargement was produced by constricting the abdominal aorta in experimental animals. The operated animals were sacrificed at different intervals after operation between...
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