Capillary Electrophoresis for Separation of Biomolecules and Viruses

Ana Gargaun
This thesis examines the use of capillary electrophoresis for the study of several biomolecules and their interactions and viruses. The first two experimental chapters focus on its utility for thermodynamic and kinetic analysis of molecules. Chapter one focuses on the use of non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM), to calculate the dissociation constant for the interaction between double stranded microRNA-122 and protein p19. NECEEM was used to calculate the rate constants (koff = 0.059...
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