Samples of the upper 5-cm topsoil were taken in spring 2019 at three sites with paired agroforestry and conventional monoculture croplands. Samples within the agroforestry systems were taken in the tree row and at 1, 7, and 24 m distance in the crop row from the tree row. We quantified 13 taxonomic groups of microorganisms and 9 genes involved in N cycling (N2 fixation, nitrification, and denitrification) in soil using real-time PCR.
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