Replication data for: A novel sweet potato potyvirus open reading frame (ORF) is expressed via polymerase slippage and suppresses RNA silencing

Milton Untiveros, Allan Olspert, Katrin Artola, Andrew Firth, Jan Kreuze & Jari Valkonen
The accumulation of GFP mRNA and GFP mRNA-derived siRNA was evaluated by northern blot analysis in leaf tissues agroinfiltrated for coexpression of GFP and P1, P1N-PISPO, SPFMV HCpro, SPLV P1, PVA HCpro or GUS. Total RNA was extracted with TRIzol LS Reagent (Invitrogen) according to the manufacturer’s recommendations alow-molecular-weight (LMW) and high-molecular-weight (HMW) RNA fractions were separated as described in Cuellar et al. (2009). LMW RNA (15 lg/lane) and HMW RNA (10 lg/lane) were separated...
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