Kerdaffrec Envel
Seeds from three biological replicates per line were pooled together to compensate for growth chamber heterogeneity. About 100 seeds per genotype were spread on wet filter paper in Petri dishes subsequently placed in moisture chambers (following protocol of Alonso-Blanco et al., 2003) that were located in a culture room set at 25°C with a long-day light regime (16 hr light, 8 hr dark). Germination rate (GR21) was determined after 7 days of incubation by scoring...
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