GR63 cold

Kerdaffrec Envel
The germination rate of seeds (percentage of germinated seeds) after-ripened for 63 days (GR63) was estimated for each genotype following standard methods (Alonso Blanco et al. 2003). Briefly, about 75 seeds per genotype were spread on wet filter paper (Whatman, ref. 1001-047) in Petri dishes (Greiner Bio-One, ref. 628102) that were subsequently placed in moisture chambers to maintain high, constant humidity (close to 99%). Moisture chambers consisted of reasonably air-tight, transparent plastic boxes (Ikea), whose...
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