Investigation of Translational Reprogramming during Transient and Stable Expression of Monoclonal Antibodies in Chinese Hamster Ovary Cells

Charlotte Godfrey &
Translational reprogramming and mRNA translation e ciency greatly in uence global protein synthesis, cell proliferation and growth; important parameters in de ning recombinant protein expression yields. Polysome pro ling is a widely-used technique to analyse mRNA transla- tion and its e ciency that provides a snapshot of ribosomes loaded on mRNA transcripts at any particular time. A higher number of polysomes present on a given mRNA suggests that the mRNA is being more heavily translated...
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