Validation of the slaughterhouse porcine heart model for ex-situ heart perfusion studies
Selma E Kaffka genaamd Dengler, Mudit Mishra, Sjoerd van Tuijl, Saskia CA de Jager, Joost PG Sluijter, Pieter A Doevendans & Niels P van der Kaaij
IntroductionTo validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts (N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals (N = 6).MethodsAll hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20–25 mmHg to achieve...
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