Development of Raman Calibration Model Without Culture Data for In-Line Analysis of Metabolites in Cell Culture Media

Risa Hara, Wataru Kobayashi, Hiroaki Yamanaka, Kodai Murayama, Soichiro Shimoda & Yukihiro Ozaki
In this study, we developed a method to build Raman calibration models without culture data for cell culture monitoring. First, Raman spectra were collected and then analyzed for the signals of all the mentioned analytes: glucose, lactate, glutamine, glutamate, ammonia, antibody, viable cells, media, and feed agent. Using these spectral data, the specific peak positions and intensities for each factor were detected. Next, according to the design of the experiment method, samples were prepared by...
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