Supplemental Material for Hoyos-Manchado et al., 2020

Rafael Hoyos-Manchado, Sergio Villa-Consuegra, Modesto Berraquero, Juan Jimenez & Victor A. Tallada
Figure S1. Representative spot test of isolated mutants. (A) Example strains of ENU-induced inactivating mutations in kanMX6 (3) and hphMX6 (2) markers were grown until mid-log phase. A single spot of each mutant is plated in regular rich medium (YES) and YES supplemented with G418 or hygromycin B to show respective resistance loss. Strains are labelled by the amino acid change indicated in table 3. (B) Five-fold serial dilutions of wild type and four temperature...
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