Supplementary information for: Rapid Identification of Genome Edited Mesenchymal Stem Cell Colonies via Cas9

Wei Jiang, Wei Lian, , , , , Zhong Huang &
Supplementary Figure 1. MSC characterization prior to the genome editing. (A) The morphology of the MSCs. (B) The MSCs expressed MSC markers, analyzed by flow cytometry. (C) The MSCs could differentiate into adipocytes, osteocytes and chondrocytes. Supplementary Figure 2. Target inserting the IL-37 into AASV1 site. (A) Donor and gRNA structures. (B) PCR characterization of genome edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR product size. (C) PCR characterization of...
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