Extending resolution within a single imaging frame.

Esley Torres-García, Raúl Pinto-Cámara, Alejandro Linares, Damián Martínez, Víctor Abonza, Eduardo Brito-Alarcón, Carlos Calcines-Cruz, Gustavo Valdés-Galindo, David Torres, Martina Jabloñski, Héctor H Torres-Martínez, José L Martínez, Haydee O Hernández, José P Ocelotl-Oviedo, Yasel Garcés, Marco Barchi, Rocco D'Antuono, Ana Bošković, Joseph G Dubrovsky, Alberto Darszon, Mariano G Buffone, Roberto Rodríguez Morales, Juan Manuel Rendon-Mancha, Christopher D Wood, Armando Hernández-García … & Adán Guerrero
The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the...
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