Identification and control for the effects of bioinformatic globin depletion on human RNA-seq differential expression analysis.

Dylan Sheerin, Francisco Lakay, Hanif Esmail, Craig Kinnear, Bianca Sansom, Brigitte Glanzmann, Robert J Wilkinson, Matthew E Ritchie & Anna K Coussens
When profiling blood samples by RNA-sequencing (RNA-seq), RNA from haemoglobin (Hgb) can account for up to 70% of the transcriptome. Due to considerations of sequencing depth and power to detect biological variation, Hgb RNA is typically depleted prior to sequencing by hybridisation-based methods; an alternative approach is to deplete reads arising from Hgb RNA bioinformatically. In the present study, we compared the impact of these two approaches on the outcome of differential gene expression analysis...
This data repository is not currently reporting usage information. For information on how your repository can submit usage information, please see our documentation.