Distinct immune- and defense-related molecular fingerprints in sepatated coelomocyte subsets of Eisenia andrei earthworms

K Bodó, D Ernszt, P Németh & P Engelmann
During phylogenesis different types of immunocytes such as amoebocytes and eleocytes have developed in earthworms to defend the host against microbial pathogens. Previously we applied a cell sorting-based approach to untangle the morphological and functional properties of these aforementioned coelomocyte subsets. In order to compare their constitutive gene expression patterns, cell-sorting was performed and followed by semiquantitative RT-PCR in the distinct, separated coelomocyte subpopulations of unmanipulated Eisenia andrei earthworms. We targeted a variety of genes...
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