Three-dimensional (3D) culture is increasingly being recognized due to its superior capacity to simulate tissue-like structures compared with two-dimensional (2D) monolayers. Although much is known about the various characteristics of 2D and 3D cultures in certain cancer types, the knowledge on other tumor types is limited. In nasopharyngeal carcinoma (NPC), although Epstein-Barr virus (EBV) is consistently present in undifferentiated NPCs, most studies have focused on EBV-negative NPC cells. In Chapter 2, 3D spheroid models of...