Longitudinal imaging of motor-evoked cortical activity before and after stroke

A. L. Allegra Mascaro, E. Conti & L. Sacconi
We recorded time series of calcium activity of cortical pyramidal neurons with wide-field fluorescence microscope. Increase in GCaMP6f fluorescence was indicative of an increase in neuronal activity. The mice were head restrained during the recording session. The field of view of the microscope allows to record cortical activity in the right hemisphere and in a portion of the left one while the mouse is performing a passive extension-active retraction of the left forelimb in a...
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