Synapse maps - confocal

A. Merchan-Perez, R. Benevides, S. Gonzalez, S. Tapia, A. Kastanauskaite, A. Muñoz, A. Santuy, J. DeFelipe, R. Rodriguez, C. Alvarez & L. Valdes
We have processed brain material from transgenic mice expressing the enhanced green fluorescent protein (EGFP) coupled with the synaptic protein PSD95 (EGFP95) in order to accomplish the automated counting process using 3D connected components of fluorescent puncta in stacks of confocal microscope images for the CA1 region of the hippocampal formation. Furthermore, we have developed a PSDs volume segmentation and quantification algorithm designed to be executed in a supercomputer in order to obtain results in...
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