Capillary Zone Electrophoresis–Based Protein Size Determination and Development of an Offline CZE/EVV-2DIR Interface Platform for Proteome-on-Chip Applications

Christian B Loeffeld
The development of an offline interface platform for continuous fraction collection from capillary zone electrophoresis (CZE) is presented. The platform is specified for Proteome-On-Chip applications that employ the protein quantification and differentiation technology Electron-Vibration-Vibration Two-Dimensional Infrared (EVV-2DIR) spectroscopy as the primary protein analysis tool. It is demonstrated that the interface platform facilitates preservation of an initial CZE separation effort and enables generation of single and multi-component tracks that are accessible to examination using EVV-2DIR spectroscopy...
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