Mechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique

Reiner Hedderich, Rolf Müller, Yasmin Greulich, Norbert Bannert, Gudrun Holland, Petra Kaiser & Rolf Reissbrodt
Colony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10 s, compared with short time hand plating (5 s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques....
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