High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts

Stefanie Hoffmann, Steffi Walter, Anne-Kathrin Blume, Stephan Fuchs, Christiane Schmidt, Annemarie Scholz & Roman G. Gerlach
The quantification of bacteria in cell culture infection models is of paramount importance for the characterization of host-pathogen interactions and pathogenicity factors involved. The standard to enumerate bacteria in these assays is plating of a dilution series on solid agar and counting of the resulting colony forming units (CFU). In contrast, the virtual colony count (VCC) method is a high-throughput compatible alternative with minimized manual input. Based on the recording of quantitative growth kinetics, VCC...
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