Investigation of heterologous expression of the non-ribosomal peptide blue pigment synthase and its activator from the nuclear genome of the model microalga Chlamydomonas reinhardtii

Manar Shlbi
The non-ribosomal peptide synthase (NRPS) blue pigment synthase (BpsA) has been shown in several heterologous hosts to mediate the production of the blue pigment indigoidine from two molecules of L-glutamine. Activation of BpsA is mediated by transfer of a coenzyme A (CoA) by a 4′-phosphopantetheinyl transferase (4′-PPTase). In this thesis, I explored heterologous co-expression of BpsA and the Pseudomonas aeruginosa 4′-PPTase (PaPcpS) and their co- localization to either cytoplasm or chloroplast stroma of the green...
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