Investigating the enzymology and molecular function of 5-methylctosine in RNA

Tennille Sibbritt
Internal nucleoside methylation of eukaryotic RNA in the form of N6-methyladenosine (m⁶A) and 5-methylcytosine (m⁵C) have been known to exist for decades, however absence of facile methods to map modified sites have limited the understanding of their role. With the availability of next-generation sequencing, these drawbacks have been overcome, revealing non-random distribution of internal methylation across different transcript biotypes. Recently, we implemented a bisulfite sequencing-based technique for transcriptome-wide detection of m⁵C (bsRNA-seq) and mapped thousands...
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