Robust 3-Dimensional Visualization of Human Colon Enteric Nervous System Without Tissue Sectioning

Khaleb D Graham, Silvia Huerta-Lopez, Rajarshi Sengupta, Archana Shenoy, Sabine Schneider, Christina M Wright, Michael Feldman, Emma Furth, Amanda Lemke, Benjamin J Wilkins, Ali Naji, Edward Doolin, Marthe Howard & Robert Heuckeroth
Details a new technique to image the enteric nervous system (ENS) and other cells that control motility in the human colon in three dimensions, by combining tissue clearing, immunohistochemistry, confocal microscopy, and quantitative image analysis.
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