mRNA electroporation of PBMC is a rapid and efficient method for immunomonitoring of T-cell responses against autologous HIV-1 strains

J. Etschel, K. Maurer, C. Hofmann, K. Eismann, S. Bergmann, J. Dörrje, N. Schaft, S.M. Müller, T. Harrer & Kompetenznetzwerk HIV/AIDS
Background: Monitoring of HIV-1-specific T-cell responses is essential for the understanding of the pathogenesis of HIV-1 infection and for the development of vaccines. Currently, mainly peptides and recombinant vaccinia vectors are used for the detection of HIV-1-specific T-cells in functional assays,[for full text, please go to the a.m. URL]