Photodamage induced by multimodal non-linear optical microscopy during brain imaging

Roberta Galli, Ortrud Uckermann, Kathrin Geiger, Gabriele Schackert, Edmund Koch, Gerald Steiner & Matthias Kirsch
Objective: Non-linear optical microscopy (NLOM) is a powerful technique that enables confocal imaging of brain tissue using two-photon fluorescence (TPF), second harmonic generation and coherent anti-Stokes Raman scattering (CARS). Several optical processes can be excited to build a multimodal image[for full text, please go to the a.m. URL]