Allele-specific qPCR for the detection of IDH1 mutations in gliomas: IDH1 mut and wt are upregulated in human gliomas whereas systemic treatment and tumor relapse do not change IDH1 gene expression

Moritz Perrech, Gabriele Röhn, Roland Goldbrunner & Marco Timmer
Objective: IDH1 mutations (mut) occur in more than 70% of WHO grade II and III gliomas and secondary glioblastomas (GBM). The most frequent mutation leads to a specific amino acid change at codon 132. Diagnostics of this mutation are based on DNA sequencing and immunohistochemistry (IHC), methods[for full text, please go to the a.m. URL]