Selection of suitable reference genes for expression studies in primary, secondary and recurrent human gliomas with respect to radio- and chemotherapy using quantitative RT-PCR

Gabriele Röhn, Arend Koch, Roland Goldbrunner & Marco Timmer
Objective: Real-time RT-PCR (RT-qPCR) is frequently used to analyse gene expression. In human astrocytomas, three studies have been performed validating reference genes, however, no systematic analysis of suitable endogenous control genes exists for recurrent tumors and under treatment conditions. Therefore,[for full text, please go to the a.m. URL]