Patterned illumination single molecule localization microscopy (piSMLM): user defined blinking regions of interest cellSTORM - Cost-effective Super-Resolution on a Cellphone using dSTORM

S.-Y. Chen, F. Bestvater, Rainer Heintzmann & Christoph Cremer
Single molecule localization microscopy (SMLM) has been established as an important super-resolution technique for studying subcellular structures with a resolution down to a lateral scale of 10 nm. Usually samples are illuminated with a Gaussian shaped beam and consequently insufficient irradiance on the periphery of the illuminated region leads to artifacts in the reconstructed image which degrades image quality. We present a newly developed patterned illumination SMLM (piSMLM) to overcome the problem of uneven illumination...
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