Fluorescence Reconstruction Microscopy Data: MDCK (20x) DIC to Nuclei (DAPI) and E-Cadherin (RFP)

Julienne LaChance & Daniel Cohen
The data provided in this DataSpace consists of sample training data to be used for Fluorescence Reconstruction Microscopy (FRM) testing. We provide a subset of the MDCK (20x magnification) dataset used in our paper, in which interested parties may find more complete information about our data collection methods. Matched pairs of DIC and fluorescent images are given. The cells stably expressed E-cadherin:RFP which enabled imaging of junctional fluorescence, while the nuclei were stained using Hoechst...
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