Increased cellular free cholesterol in macrophage-specific Abca1 knock-out mice enhances pro-inflammatory response of macrophages

Xuewei Zhu, Ji-Young Lee, Jenelle M. Timmins, J. Mark Brown, Elena Boudyguina, Anny Mulya, Abraham K. Gebre, Mark C. Willingham, Elizabeth M. Hiltbold & Nilamadhab Mishra
Macrophage-specific Abca1 knock-out (Abca1(-M/-M)) mice were generated to determine the role of macrophage ABCA1 expression in plasma lipoprotein concentrations and the innate immune response of macrophages. Plasma lipid and lipoprotein concentrations in chow-fed Abca1(-M/-M) and wild-type (WT) mice were indistinguishable. Compared with WT macrophages, Abca1(-M/-M) macrophages had a > 95% reduction in ABCA1 protein, failed to efflux lipid to apoA-I, and had a significant increase in free cholesterol (FC) and membrane lipid rafts without induction...
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