Growth of Epithelial Organoids in a Defined Hydrogel

Nicolas Broguiere, Luca Isenmann, Christian Hirt, Till Ringel, Silja Placzek, Emma Cavalli, Femke Ringnalda, Lukas Villiger, Richard Züllig, Roger Lehmann, Gerhard Rogler, Markus H. Heim, Julia Schüler, Marcy Zenobi-Wong & Gerald Schwank
Epithelial organoids are simplified models of organs grown in vitro from embryonic and adult stem cells. They are widely used to study organ development and disease, and enable drug screening in patient‐derived primary tissues. Current protocols, however, rely on animal‐ and tumor‐derived basement membrane extract (BME) as a 3D scaffold, which limits possible applications in regenerative medicine. This prompted us to study how organoids interact with their matrix, and to develop a well‐defined hydrogel that...
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