HA-RNA polymerase II ChIP-seq in RAJI cells

Romain Fenouil, Frédéric Koch, Jean-Christophe Andrau & Pierre Ferrier
This experiment was to assess total RNA pol II genome-wide binding locations in immortalized B lymphocyte in culture. The ChIP was performed with an anti-HA antibody that is fused to the largest subunit of Pol II in order to facilitate the IP procedure.

1 Related Work

Deciphering Transcriptional Regulatory Networks that Control Differentiation, Identity and Proliferation of Murine ES and T Cells

Pierre Ferrier & Jean-Christophe Andrau
We are interested in transcription factor binding patterns both in murine ES cells and in differentiated T cells. Among others we study genome wide recruitment of RNA poymerase II, TBP, polycomb and other TFs in these cells using either chip on chip or ChIPseq approaches.

Resource Types

  • Text

Publication Year

  • 2010

Data Centers

  • INIST/CNRS - Standard-Based Infrastructure with Distributed Resources