This experiment was to assess total RNA pol II genome-wide binding locations in immortalized B lymphocyte in culture. The ChIP was performed with an anti-HA antibody that is fused to the largest subunit of Pol II in order to facilitate the IP procedure.
1 Related Work
Deciphering Transcriptional Regulatory Networks that Control Differentiation, Identity and Proliferation of Murine ES and T CellsPierre Ferrier & Jean-Christophe Andrau
We are interested in transcription factor binding patterns both in murine ES cells and in differentiated T cells. Among others we study genome wide recruitment of RNA poymerase II, TBP, polycomb and other TFs in these cells using either chip on chip or ChIPseq approaches.