Effect of sulfated oligosaccharides (heparin and fucoidan) on the oligomerization of stromal cell-derived factor-1

Soraya Fermas, Florence Gonnet, Angela Sutton, Nathalie Charnaux, Barbara Mulloy, Yuguo Du, Françoise Baleux & Régis Daniel
We investigate the dimerization of SDF-1 in microM concentration and in the presence of sulfated oligosaccharides, using affinity capillary electrophoresis (ACE) hyphenated to electrospray ionization-mass spectrometry (ESI/MS). Chemokine stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant involved in leukocyte trafficking and metastasis. Heparan sulfate on the cell surface binds SDF-1 and may modulate its function as a coreceptor of this chemokine. A major effect of the glycosaminoglycan binding may be on the quaternary structure of...