Redefining the Functional Repertoire and Metal Dependence of Type I Cas3 HD Domain Nucleases

Paul Yanning Jiang
Targeted DNA degradation by the Type I CRISPR-Cas system is performed by Cas3, typically a two-component protein with a nuclease and a helicase domain. The nuclease belongs to the HD-domain metalloprotein superfamily, that harbors functionally diverse representatives including phosphodiesterases, phosphatases and oxygenases. The range of metal ions reported to support Cas3 catalytic activity is diverse, including Mg, Mn, and Ni, demonstrating variability in cofactor requirements. Type IE Cas3 nucleases are dinuclear, whereas for Type IA...
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