Data from: DNA extraction method affects the detection of a fungal pathogen in formalin-fixed specimens using qPCR

Andrea J. Adams, John P. LaBonte, Morgan L. Ball, Kathryn L. Richards-Hrdlicka, Mary H. Toothman & Cheryl J. Briggs
Museum collections provide indispensable repositories for obtaining information about the historical presence of disease in wildlife populations. The pathogenic amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) has played a significant role in global amphibian declines, and examining preserved specimens for Bd can improve our understanding of its emergence and spread. Quantitative PCR (qPCR) enables Bd detection with minimal disturbance to amphibian skin and is significantly more sensitive to detecting Bd than histology; therefore, developing effective qPCR...
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