Data from: Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments

Tae Yeon Yoo, Jeong-Mo Choi, William Conway, Che-Hang Yu, Rohit V. Pappu & Daniel J. Needleman
Proper kinetochore-microtubule attachments, mediated by the NDC80 complex, are required for error-free chromosome segregation. Erroneous attachments are corrected by the tension dependence of kinetochore-microtubule interactions. Here, we present a method, based on fluorescence lifetime imaging microscopy and Förster resonance energy transfer, to quantitatively measure the fraction of NDC80 complexes bound to microtubules at individual kinetochores in living human cells. We found that NDC80 binding is modulated in a chromosome autonomous fashion over prometaphase and metaphase,...
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