Quantifying and reducing cross-contamination in single- and multiplex hybridization capture of ancient DNA

Elena Zavala, Ayinuer Aximu-Petri, Julia Richter, Birgit Nickel, Benjamin Vernot & Matthias Meyer
The use of hybridization capture has enabled a massive upscaling in sample sizes for ancient DNA studies, allowing the analysis of hundreds of skeletal remains (Mathieson et al., 2015; Narasimhan et al., 2019) or sediments (Vernot et al., 2021; Wang et al., 2021; Zavala et al., 2021) in single studies. Yet demands in throughput continue to grow, and hybridization capture has become a limiting step in sample preparation due to the large consumption of reagents,...
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