Data from: Validation of COI metabarcoding primers for terrestrial arthropods

Vasco Elbrecht, Thomas W. A. Braukmann, Natalia V. Ivanova, Sean W. J. Prosser, Mehrdad Hajibabaei, Michael Wright, Evgeny V. Zakharov, Paul D. N. Hebert & Dirk Steinke
Metabarcoding can rapidly determine the species composition of bulk samples and thus aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that minimize amplification bias among taxa to maximize species recovery. Despite this fact, the performance of primer sets employed for metabarcoding terrestrial arthropods has not been sufficiently evaluated. This study tests the performance of 36 primer sets on a mock community containing 374 insect species. Amplification success was assessed with...
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