Data from: One-locus-several-primers: a strategy to improve the taxonomic and haplotypic coverage in diet metabarcoding studies

Emmanuel Corse, Christelle Tougard, Gaït Archambaud-Suard, Jean-François Agnèse, Françoise D. Messu Mandeng, Charles F. Bilong Bilong, David Duneau, Lucie Zinger, Rémi Chappaz, Charles C. Y. Xu, Emese Meglécz & Vincent Dubut
In diet metabarcoding analyses, insufficient taxonomic coverage of PCR primer sets generates false negatives that may dramatically distort biodiversity estimates. In this paper, we investigated the taxonomic coverage and complementarity of three cytochrome c oxidase subunit I gene (COI) primer sets based on in silico analyses and we conducted an in vivo evaluation using fecal and spider web samples from different invertivores, environments, and geographic locations. Our results underline the lack of predictability of both...
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These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
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