Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'

Stephan Riesenberg, Lukas Bokelmann, Tomislav Maricic, Svante Pääbo, Matthias Meyer, Olaf Nickel & Stephan Borte
Here we present images of loop-mediated isothermal amplification (LAMP) and optimized Cap-iLAMP (capture and improved ‎loop-mediated isothermal amplification). Cap-iLAMP combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. The sensitivity is 97% and the specificity is 99%.
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