Data from: Optimization of the genotyping-by-sequencing strategy for population genomic analysis in conifers

Jin Pan, Baosheng Wang, Zhi-Yong Pei, Wei Zhao, Jie Gao, Jian-Feng Mao & Xiao-Ru Wang
Flexibility and low cost make genotyping-by-sequencing (GBS) an ideal tool for population genomic studies of nonmodel species. However, to utilize the potential of the method fully, many parameters affecting library quality and single nucleotide polymorphism (SNP) discovery require optimization, especially for conifer genomes with a high repetitive DNA content. In this study, we explored strategies for effective GBS analysis in pine species. We constructed GBS libraries using HpaII, PstI and EcoRI-MseI digestions with different multiplexing...
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