Data from: Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

Rohan Gala, Daniel Lebrecht, Daniela A. Sahlender, Anne Jorstad, Graham Knott, Anthony Holtmaat & Armen Stepanyants
The ability to measure minute structural changes in neural circuits is essential for long-term in vivo imaging studies. Here, we propose a methodology for detection and measurement of structural changes in axonal boutons imaged with time-lapse two-photon laser scanning microscopy (2PLSM). Correlative 2PLSM and 3D electron microscopy (EM) analysis, performed in mouse barrel cortex, showed that the proposed method has low fractions of false positive/negative bouton detections (2/0 out of 18), and that 2PLSM-based bouton...
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These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
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