Data from: Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities

Nicholas K. M. Lim, Ywee Chieh Tay, Amrita Srivathsan, Jonathan W. T. Tan, Jeffrey T. B. Kwik, Bilgenur Baloğlu, Rudolf Meier & Darren C. J. Yeo
Freshwater habitats are of high conservation value and provide a wide range of ecosystem services. Effective management requires regular monitoring. However, conventional methods based on direct observation or specimen collection are so invasive, expensive and labour-intensive that frequent monitoring is uncommon. Here, we test whether the evaluation of environmental DNA (eDNA) from water based on a simple protocol can be used for assessing biodiversity. We use universal metazoan primers for characterizing water eDNA across horizontal...
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