In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture

Shannon Klein, Samhan Alsolami, Silvia Arossa, Gerardo Ramos Mandujano, Anieka Parry, Alexandra Steckbauer, Carlos Duarte & Mo Li
Mammalian cell cultures are a keystone resource in biomedical research, but the results of published experiments often suffer from reproducibility challenges. This has led to a focus on the influence of cell culture conditions on cellular responses and reproducibility of experimental findings. Here, we perform frequent in situ monitoring of dissolved O2 and CO2 with optical sensor spots and contemporaneous evaluation of cell proliferation and medium pH in standard batch cultures of three widely used...
1 citation reported since publication in 2021.
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