Data from: A sequel to Sanger: amplicon sequencing that scales

Paul D.N. Hebert, Thomas W.A. Braukmann, Sean W.J. Prosser, Sujeevan Ratnasingham, Jeremy R. DeWaard, Natalia V. Ivanova, Daniel H. Janzen, Winnie Hallwachs, Suresh Naik, Jayme E. Sones & Evgeny V. Zakharov
Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) sequencing implemented on the SEQUEL platform to overcome these limitations, employing 658 bp amplicons of the mitochondrial cytochrome c oxidase I gene as a model system. By examining templates from more than 5,000 species and 20,000 specimens,...
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