Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river

Naiara Sales, Owen S. Wangensteen, Daniel Carvalho & Stefano Mariani
Environmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be a sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limitations remain to be addressed. We investigated the effect of sampling me‐ dium and time, and preservation methods, on fish detection performance based on eDNA metabarcoding of neotropical freshwater samples. Water and sediment sam‐ ples were collected from 11 sites along the...
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