Data from: “Direct PCR” optimization yields a rapid, cost-effective, non-destructive, and efficient method for obtaining DNA barcodes without DNA extraction

Wing Hing Wong, Ywee Chieh Tay, Jayanthi Puniamoorthy, Michael Balke, Peter S. Cranston & Rudolf Meier
Macroinvertebrates that are collected in large numbers pose major problems in basic and applied biodiversity research: identification to species via morphology is often difficult, slow, and/or expensive. DNA barcodes are an attractive alternative or complementary source of information. Unfortunately obtaining DNA barcodes from specimens requires many steps and thus time and money. Here, we promote a short-cut to DNA barcoding; i.e., a non-destructive PCR method that skips DNA extraction (“direct PCR”) and that can be...
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These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?